Electron spin resonance
Electron spin resonance (ESR) or electron paramagnetic resonance spectroscopy (EPR) is a sophisticated spectroscopic technique that detects free radicals or paramagnetic metals in chemical and biological systems. Generally, these radicals are unstable, but when they are first reacted with spin-trapping reagents, they can be trapped and stabilized. On the other hand, with use of a spin-labeling reagent, the molecules without radicals can also be measured with ESR/EPR.
Our lab is equipped with state-of-the-art commercial EPR spectrometers as well as with unique home built instruments
Bruker Continuous Wave and Puls EleXsys E580 EPR-ELDOR Spectrometer
Pulse Bruker EPR spectrometer is equipped with X-band (9GHz) and Super-Q-FTu – EPR (35GHz) microwave bridges and set of resonators.
Variable temperature capabilities from 4K to 370K using Oxford Instruments He system, He transfer line and Oxford Instruments gas flow and temperature controllers.
Bruker Continuous Wave EleXsys E500 EPR Spectrometer
CW Bruker EPR spectrometer with SuperX feature for high sensitivity experiments with aqueous samples is well suited for protein spin-labeling experiments
VariaN X-BAND EPR SPECTROMETER
CW spectrometer that is easy to use for everyday measurements with variable temperature capabilities from liquid nitrogen to +60 C. Unique home-build variable temperature system for biophysical measurements in temperature range from -5 C to +60 C offers remarkable temperature stability of 0.02 C. Everyone’s favorite old friend!
High Field EPR Instrumentation
2 T cryogen-free wide bore superconductive magnet with +/500 G sweep coil and 10 ppm homogeneity in 1 cm sphere (Cryogenic Ltd, UK).
9 T cryogen-free wide bore superconductive magnet with +/500 G sweep coil and 10 ppm homogeneity in 1 cm sphere (Cryogenic Ltd, UK).
CW W-band (3 mm wave length, 95 GHz) home-build EPR bridge with liquid Helium accessory (CryoIndustries of America, NH)
CW/pulsed D-band (2 mm wave length, 130 GHz) heterodyne high power (130 mW from two channels) three-channel microwave bridge (HF EPR Instruments, Brooklyn, NY).
Cryogen-free dilution refrigerator capable of base temperatures down to 12-20 mK (demonstrated) in a 9 T magnetic field (Leiden Cryogenic, Netherlands). Wide bore superconductive magnet with +/500 G sweep coil and 10 ppm homogeneity in 1 cm sphere (Cryogenic Ltd, UK).